研制了一种用于体内成像诊断和光动力学治疗的荧光显微内窥术，可根据探测的荧光信号强度，采用反馈算法快速调节不同成像区域的激发光强度，获取高信噪比、大动态范围的荧光图像, 实现交互式荧光诊断与光动力学治疗分析。根据反馈的治疗效果，自适应地连续实时调整光剂量，获得最佳的光动力学治疗结果。激发光强度的动态调整由计算机控制数字微镜器件的开关状态及驻留时间来实现。荧光显微内窥镜的外径尺寸为8 mm，工作长度为250.3 mm，可装配在腹腔镜管鞘中，适用于体内病灶的显微成像诊断。实验结果表明，显微成像视场为600 μm，光学分辨率优于2.2 μm，图像动态范围可增强200倍以上，有效改善了荧光微弱区域和荧光饱和区域的图像细节和信噪比，提高了诊断准确性。此技术可进一步扩展为共焦三维成像，实现在体组织细胞学分析。

当相干光在粗糙表面散射时,会产生散斑现象。散斑严重影响了图像质量,必须加以抑制。通过散射体振动产生一系列的散斑图样,并在传感器积分时间内加以平均,可以达到抑制散斑的效果。将此方法应用于基于硅基液晶(LCoS)芯片的激光投影系统,并通过一系列实验研究了散斑对比度和散射体运动之间的关系。散斑抑制后,传感器上的散斑对比度从0.49降低到了0.065。实验表明散射体更快更大的振动和更小的单元尺寸都可以降低散斑对比度。

A novel approach has been proved to quickly and non-invasively determine the optical properties of human skin in vivo. It is based on the diffuse reflectance approximation model and subjected to the well established library of absorption spectra of water and hemoglobin. Under the nonlinear least-square algorithm, fitting the measured spectra in the range of 400-1000 nm to the diffusion approximation model, the reduced scattering coefficient and absorption coefficient of skin tissue can be quickly determined in vivo. The results show that this method is convenient and suitable for the real-time clinical application.

With improvements in infrared camera technology and the promise of reduced costs and noninvasive character, infrared thermal imaging resurges in medicine. The skin temperature distribution of a healthy human body exhibits a contralateral symmetry. Any disease inside body is associated with an alteration of the thermal distribution of human body. So human thermography is of unique value in studying the physiology of thermoregulation, diagnosing some diseases or evaluating the situation of human health. The physical principle of human thermography and its clinical applications are dicsussed. The latest progress of thermography, thermal texture maps (TTM) and its effectiveness are introduced.

Cross-talk phenomenon in dual-labeled fluorescent microarray scanning is analyzed from cross-excitation and cross-emission. It is turned out that the spectral overlap of the fluorophores is crucial for cross-talk error, and this error can be corrected by an image subtraction method. The experiment was successfully applied to separate the Cy3 channel and the Cy5 channel in microarray scanning. The cross-talk error was reduced from more than 1% to about 0.1%.

Protein microarray technology has recently emerged as a powerful tool for biomedical research. Before automatic analysis the protein microarray images, protein spots in the images must be determined appropriately by spot segmentation algorithm. In this paper, an improved seeded region growing (ISRG) algorithm for protein microarray segmentation is presented, the seeds are obtained by finding the positions of the printed spots, and the protein spot regions are grown through these seeds. The experiment results show that the presented algorithm is accurate for adaptive shape segmentation and robust for proteinmicroarray images contaminated by noise.

A new wavelength beam combining technique for a high-power laser diode bar by using a temperature gradient heat sink has been proposed. The thermal controlling principle of the temperature heat sink has been discussed. It has been proved by experiment that the linear temperature distribution, which generates linear wavelength spread of the output beams from a LD bar, can be obtained by introducing a temperature gradient heat sink and the output beams can be focused into a relative small spot by using the Czerny-Turner beam shaping system.